Journal: The Journal of Neuroscience

Article Title: miR126-5p Downregulation Facilitates Axon Degeneration and NMJ Disruption via a Non–Cell-Autonomous Mechanism in ALS

doi: 10.1523/JNEUROSCI.3037-17.2018

Figure Lengend Snippet: miR126-5p is depleted in SOD1 G93A muscles and regulates Sema3 and NRP expression. A , NanoString chip screen heat map of significantly altered miRs in P60 muscles of SOD1 G93A compared with LM mice (extended table under ). Red and green represent a high or low abundance of miRs, respectively. * p < 0.05 (Student's t test; n = 3). B , miR126-5p was the most significantly downregulated miRNA in SOD1 G93A muscles (Student's t test; n = 3, ** p = 0.003). C , qPCR analysis of P60 GC muscle extracts further validates the decrease in miR 126-5p in SOD1 G93A ( n = 3). D–G , qPCR analysis of Sema3A, NRP1, Sema3B, and NRP2 transcript levels in HeLa cells overexpressing either miR126-5p or miR142 demonstrates a reduction in their expression levels specifically under miR126-5p overexpression (Student's t test; n = 3, * p = 0.0438, * p = 0.034, * p = 0.031, and * p = 0.0434, respectively). H , Representative TIRF images of U87MG cells reveal a detachment of the cell membrane from the culture dish surface after Sema3A is added to the culture medium. Scale bar, 10 μm. I , Impedance recording of live cells over time shows that U87MG cells overexpressing miR126-5p are unresponsive to Sema3A added to the culture medium because their impedance continuously increases, whereas the impedance of U87MG cells overexpressing miR142 decreases after treatment .

Article Snippet: Antibodies were used at the following concentrations: anti-Neurofilament Heavy Chain 1:500 (NFH, Abcam, ab72996; 1:1000), synaptophysin (Millipore, MAB5258; 1:300), synaptotagmin (Alomone Labs, ant-003; 1:300), anti-NRP1 (1:100), anti-Sema3A (1:100), anti-NRP2 (1:100), and anti-Sema3B (1:100).

Techniques: Expressing, Over Expression

Journal: International Journal of General Medicine

Article Title: Effects of Sintilimab Plus Radiotherapy on Levels of Spondin-2 and Glucose Transporter-1 in Patients with Cervical Cancer

doi: 10.2147/IJGM.S461606

Figure Lengend Snippet: Levels of Serum Tumor Markers Before and After Six Cycles of Treatment ( \documentclass[12pt]{minimal} \usepackage{wasysym} \usepackage[substack]{amsmath} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage[mathscr]{eucal} \usepackage{mathrsfs} \DeclareFontFamily{T1}{linotext}{} \DeclareFontShape{T1}{linotext}{m}{n} {linotext }{} \DeclareSymbolFont{linotext}{T1}{linotext}{m}{n} \DeclareSymbolFontAlphabet{\mathLINOTEXT}{linotext} \begin{document} $\bar x\pm s$\end{document} )

Article Snippet: Then the levels of carcinoembryonic antigen (CEA), squamous cell carcinoma antigen (SCC-Ag), vascular endothelial growth factor-A (VEGF-A) and vascular endothelial growth factor receptor 2 (VEGFR2) were measured by enzyme-linked immunosorbent assay (ELISA) in strict accordance with the instructions of kits provided by Wuhan CUSABIO Co., Ltd. (China) and Shanghai Jining Biological Preparation Co., Ltd. (China). (4) Levels of Spondin-2 and Glut-1: The levels of serum Spondin-2 and Glut-1 were measured by ELISA before treatment and after six cycles of treatment. (5) Survival status: All patients were followed up by outpatient visit or telephone calls for 18 months to record their survival status.

Techniques: Control

Journal: Journal of Neuroinflammation

Article Title: Succinate-induced macrophage polarization and RBP4 secretion promote vascular sprouting in ocular neovascularization

doi: 10.1186/s12974-023-02998-1

Figure Lengend Snippet: Effect of RBP4 on sprouting in vivo and in vitro. HUVECs were treated with 10 ng/ml RBP4 for 48 h, with or without 1 μM Ki8751 pretreatment for 12 h. A Relative mRNA expression of ANGPT2, TIE1, HEY1 and DLL4 in RBP4-treated HUVECs ( n = 3). B Relative mRNA expression of ANGPT2, TIE1, HEY1 and DLL4 in the control, RBP4 and RBP4 + Ki8751 groups ( n = 3). C , D Protein levels of VEGFR2 in the three groups ( n = 3). E Immunofluorescence staining of DAPI (blue) and VEGFR2 (red) in HUVECs. Scale bar: 50 μm. F Co-IP examination of VEGFR2-RBP4 interaction. H , J , K Retinal IB4 staining of P14 in OIR and relative number of tip cells as well as filopodia. Scale bar: 200 μm ( n = 3). G , I Choroidal sprouting analysis in three groups ( n = 3). Scale bar: 500 μm. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: Administration of the VEGFR2 inhibitor Ki8751 (1 μM for 12 h, #HY-12038, MCE) reduced VEGFR2 expression and attenuated RBP4 promotion of ANGPT2 and TIE1 as well as inhibition of HEY1 and DLL4 (Fig. B).

Techniques: In Vivo, In Vitro, Expressing, Control, Immunofluorescence, Staining, Co-Immunoprecipitation Assay

Journal: Arthritis Research & Therapy

Article Title: Semaphorin 3G exacerbates joint inflammation through the accumulation and proliferation of macrophages in the synovium

doi: 10.1186/s13075-022-02817-7

Figure Lengend Snippet: Nrp2 expression in activated macrophages during joint inflammation. A Nrp2 expression in immune cells in the CIA synovium. The hind paws of CIA-induced mice were digested and subjected to flow-cytometric analysis. The representative histograms of Nrp2 staining and the cumulative data are shown. Data are expressed as the means ± SD. N = 3 from three independent experiments. B The characteristics of Nrp2-high macrophages. The representative histograms of CD86 and MHC class II expression on joint-infiltrating macrophages (left) and their cumulative data (right) are shown. N = 6 from two independent experiments. C Nrp2 expression on BMMs. BMMs were cultured under the indicated conditions, and Nrp2 expression was determined by flow cytometry. The representative histograms and the cumulative data are shown. N = 3 from three independent experiments. D NRP2 expression in synovium-infiltrating cells in RA. The deposited single-cell RNA-seq data were reanalyzed, and several cell types were defined by UMAP. NRP2 expression is denoted by purple dots. The statistical analyses were performed using an unpaired t-test

Article Snippet: Anti-neuropilin2 (Nrp2) polyclonal antibodies conjugated with FITC were purchased from Alomone Labs (#ANR-062).

Techniques: Expressing, Staining, Cell Culture, Flow Cytometry, RNA Sequencing